2vwj

X-ray diffraction
2.78Å resolution

Uracil Recognition in Archaeal DNA Polymerases Captured by X-ray Crystallography.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 773 amino acids
Theoretical weight: 89.94 KDa
Source organism: Thermococcus gorgonarius
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P56689 (Residues: 1-773; Coverage: 100%)
Gene names: pol, polA
Sequence domains:
Structure domains:
5'-D(*AP*AP*UP*GP*GP*AP*GP*AP*CP*GP *GP*CP*TP*TP*TP*TP*GP*CP*CP*GP*TP*GP*TP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 26 nucleotides
Theoretical weight: 8.01 KDa
Source organism: Thermococcus gorgonarius
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: I222
Unit cell:
a: 100.7Å b: 161.91Å c: 221.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.264
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided