PDBe 2vs7

X-ray diffraction
2.05Å resolution

The crystal structure of I-DmoI in complex with DNA and Ca

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homing endonuclease I-DmoI Chains: A, D, G
Molecule details ›
Chains: A, D, G
Length: 199 amino acids
Theoretical weight: 23.27 KDa
Source organism: Desulfurococcus mucosus
Expression system: Escherichia coli
UniProt:
  • Canonical: P21505 (Residues: 2-190; Coverage: 97%)
Sequence domains: LAGLIDADG-like domain
Structure domains: Homing endonucleases
5'-D(*GP*CP*CP*TP*TP*GP*CP*CP*GP*GP *GP*TP*AP*AP*GP*TP*TP*CP*CP*GP*GP*CP*GP*CP*G)-3' Chains: B, E, H
Molecule details ›
Chains: B, E, H
Length: 25 nucleotides
Theoretical weight: 7.71 KDa
Source organism: Desulfurococcus mucosus
Expression system: Not provided
5'-D(*CP*GP*CP*GP*CP*CP*GP*GP*AP*AP *CP*TP*TP*AP*CP*CP*CP*GP*GP*CP*AP*AP*GP*GP*C)-3' Chains: C, F, I
Molecule details ›
Chains: C, F, I
Length: 25 nucleotides
Theoretical weight: 7.65 KDa
Source organism: Desulfurococcus mucosus
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 106.988Å b: 70.587Å c: 106.848Å
α: 90° β: 119.75° γ: 90°
R-values:
R R work R free
0.189 0.187 0.228
Expression systems:
  • Escherichia coli
  • Not provided