2vqu

X-ray diffraction
1.9Å resolution

Structural and biochemical evidence for a boat-like transition state in beta-mannosidases

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183790 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-mannosidase B Chains: A, B
Molecule details ›
Chains: A, B
Length: 846 amino acids
Theoretical weight: 97.87 KDa
Source organism: Bacteroides thetaiotaomicron
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8AAK6 (Residues: 26-864; Coverage: 100%)
Gene name: BT_0458
Sequence domains:
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 91.1Å b: 115.1Å c: 99.5Å
α: 90° β: 113.4° γ: 90°
R-values:
R R work R free
0.179 0.177 0.231
Expression system: Escherichia coli BL21