› 2vpj

X-ray diffraction
1.85Å resolution

Crystal structure of the Kelch domain of human KLHL12

Released:
DOI: 10.2210/pdb2vpj/pdb
PDB 2vpj coloured by chain and viewed from the front.
PDB 2vpj coloured by chain and viewed from the side.
PDB 2vpj coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Structural basis for Cul3 protein assembly with the BTB-Kelch family of E3 ubiquitin ligases.
Canning P, Cooper CDO, Krojer T, Murray JW, Pike ACW, Chaikuad A, Keates T, Thangaratnarajah C, Hojzan V, Marsden BD, Gileadi O, Knapp S, von Delft F, Bullock AN
OpenAccess logo J Biol Chem 288 7803-7814 (2013)
PMID: 23349464

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kelch-like protein 12 Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 32.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53G59 (Residues: 267-567; Coverage: 53%)
Gene names: C3IP1, KLHL12
Structure domains: Kelch-type beta propeller

Ligands and Environments

1 bound ligand:
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 44.637Å b: 61.546Å c: 45.523Å
α: 90° β: 111.75° γ: 90°
R-values:
R R work R free
0.164 0.161 0.222
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

29 review citations

Potential Applications of NRF2 Modulators in Cancer Therapy.
Panieri et al. (2020)
28 more

8 mentions without citation

Drug resistance genomics of the antimalarial drug artemisinin.
Winzeler et al. (2014)
7 more