2vnt

X-ray diffraction
2.2Å resolution

UROKINASE-TYPE PLASMINOGEN ACTIVATOR INHIBITOR COMPLEX WITH A 1-(7- SULPHOAMIDOISOQUINOLINYL)GUANIDINE

Released:

Function and Biology Details

Reaction catalysed:
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Urokinase-type plasminogen activator chain B Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 276 amino acids
Theoretical weight: 31.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00749 (Residues: 156-431; Coverage: 67%)
Gene name: PLAU
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS
Spacegroup: C2
Unit cell:
a: 104.557Å b: 181.108Å c: 104.363Å
α: 90° β: 94.8° γ: 90°
R-values:
R R work R free
0.244 0.241 0.295
Expression system: Escherichia coli