2vla

X-ray diffraction
1.3Å resolution

Crystal structure of restriction endonuclease BpuJI recognition domain in complex with cognate DNA

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Restriction endonuclease R.BpuJI Chain: A
Molecule details ›
Chain: A
Length: 285 amino acids
Theoretical weight: 33.48 KDa
Source organism: Bacillus pumilus
Expression system: Escherichia coli
UniProt:
  • Canonical: A3FMN7 (Residues: 1-285; Coverage: 62%)
Gene name: bpuJIR
Sequence domains: Restriction endonuclease BpuJI - N terminal
Structure domains:
5'-D(*GP*GP*TP*AP*CP*CP*CP*GP*TP*GP *GP*A)-3' Chain: L
Molecule details ›
Chain: L
Length: 12 nucleotides
Theoretical weight: 3.7 KDa
Source organism: Bacillus pumilus
Expression system: Not provided
5'-D(*TP*CP*CP*AP*CP*GP*GP*GP*TP*AP *CP*C)-3' Chain: M
Molecule details ›
Chain: M
Length: 12 nucleotides
Theoretical weight: 3.62 KDa
Source organism: Bacillus pumilus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: P21212
Unit cell:
a: 56.292Å b: 167.513Å c: 43.846Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.138 0.166
Expression systems:
  • Escherichia coli
  • Not provided