› 2vg8

X-ray diffraction
1.75Å resolution

Characterization and engineering of the bifunctional N- and O- glucosyltransferase involved in xenobiotic metabolism in plants

Released:
DOI: 10.2210/pdb2vg8/pdb
PDB 2vg8 coloured by chain and viewed from the front.
PDB 2vg8 coloured by chain and viewed from the side.
PDB 2vg8 coloured by chain and viewed from the top.
Source organism: Arabidopsis thaliana
Entry authors: Brazier-Hicks M, Offen WA, Gershater MC, Revett TJ, Lim EK, Bowles DJ, Davies GJ, Edwards R

Function and Biology Details

Reaction catalysed: 
UDP-glucose + hydroquinone = UDP + hydroquinone-O-beta-D-glucopyranoside
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glycosyltransferase 72B1 Chain: A
Molecule details ›
Chain: A
Length: 480 amino acids
Theoretical weight: 52.99 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9M156 (Residues: 1-480; Coverage: 100%)
Gene names: At4g01070, F2N1.15, UGT72B1
Sequence domains: UDP-glucoronosyl and UDP-glucosyl transferase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

3 bound ligands:
Ligand UDP 1 x UDP
Ligand TRS 1 x TRS
Ligand EDO 9 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 47.642Å b: 94.418Å c: 56.676Å
α: 90° β: 110.35° γ: 90°
R-values:
R R work R free
0.173 0.171 0.209
Expression system: Escherichia coli

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Citations

22 review citations

Computer Simulation to Rationalize "Rational" Engineering of Glycoside Hydrolases and Glycosyltransferases.
Coines et al. (2022)
21 more

3 mentions without citation

Characterization and engineering of the bifunctional N- and O-glucosyltransferase involved in xenobiotic metabolism in plants.
Brazier-Hicks et al. (2007)
2 more