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Structural model for the complex between the Dr adhesins and carcinoembryonic antigen (CEA)

Solution NMR
PDB entry 2ver coloured by chain, front view.
PDB entry 2ver coloured by chain, side view.
PDB entry 2ver coloured by chain, top view.
Source organisms:
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Carcinoembryonic antigen-related cell adhesion molecule 5 and Afimbrial adhesin AFA-III
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Multimeric state: hetero dimer
Accessible surface area: 12213.9 Å2
Buried surface area: 2363.83 Å2
Dissociation area: 813.61 Å2
Dissociation energy (ΔGdiss): -4.34 kcal/mol
Dissociation entropy (TΔSdiss): 11.27 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-139184

Macromolecules

Afimbrial adhesin AFA-III
Chain: A
Length: 143 amino acids
Theoretical weight: 15.46 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q57254 (Residues: 38-160; Coverage: 89%)
Gene names: afaE-3, afaE3
Pfam: Dr-family adhesin
InterPro:
CATH: Dr adhesin
SCOP: Dr-family adhesin
Afimbrial adhesin AFA-III in PDB entry 2ver, assembly 1, front view.
Afimbrial adhesin AFA-III in PDB entry 2ver, assembly 1, side view.
Afimbrial adhesin AFA-III in PDB entry 2ver, assembly 1, top view.
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Carcinoembryonic antigen-related cell adhesion molecule 5
Chain: N
Length: 110 amino acids
Theoretical weight: 12.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P06731 (Residues: 35-144; Coverage: 17%)
Gene names: CEA, CEACAM5
Pfam: Immunoglobulin V-set domain
InterPro:
CATH: Immunoglobulins
Carcinoembryonic antigen-related cell adhesion molecule 5 in PDB entry 2ver, assembly 1, front view.
Carcinoembryonic antigen-related cell adhesion molecule 5 in PDB entry 2ver, assembly 1, side view.
Carcinoembryonic antigen-related cell adhesion molecule 5 in PDB entry 2ver, assembly 1, top view.
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