PDBe 2ve7

X-ray diffraction
2.88Å resolution

Crystal structure of a bonsai version of the human Ndc80 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kinetochore protein NDC80 homolog; Kinetochore protein Spc25 Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 36.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HBM1 (Residues: 118-224; Coverage: 48%)
  • Canonical: O14777 (Residues: 80-286; Coverage: 32%)
  • nullnull
Gene names: AD024, HEC, HEC1, KNTC2, NDC80, SPBC25, SPC25
Sequence domains:
Structure domains: Actin-binding Protein, T-fimbrin; domain 1
Kinetochore protein Nuf2; Kinetochore protein Spc24 Chains: C, D
Molecule details ›
Chains: C, D
Length: 250 amino acids
Theoretical weight: 28.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BZD4 (Residues: 1-169; Coverage: 36%)
  • Canonical: Q8NBT2 (Residues: 122-197; Coverage: 39%)
  • nullnull
Gene names: CDCA1, NUF2, NUF2R, SPBC24, SPC24
Sequence domains: Nuf2 family
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 157.653Å b: 248.965Å c: 58.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.232 0.261
Expression system: Escherichia coli BL21(DE3)