X-ray diffraction
2.3Å resolution

Structure of trm8, m7G methylation enzyme


Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
tRNA (guanine-N(7)-)-methyltransferase Chains: E, F
Molecule details ›
Chains: E, F
Length: 246 amino acids
Theoretical weight: 28.9 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
  • Canonical: Q12009 (Residues: 47-286; Coverage: 84%)
Gene names: D1075, TRM8, YDL201W
Sequence domains: Putative methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

Cofactor: Ligand SAM 2 x SAM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 128.318Å b: 58.686Å c: 94.76Å
α: 90° β: 130.13° γ: 90°
R R work R free
0.233 0.233 0.265
Expression system: Escherichia coli