2vce

X-ray diffraction
1.9Å resolution

Characterization and engineering of the bifunctional N- and O- glucosyltransferase involved in xenobiotic metabolism in plants

Released:

Function and Biology Details

Reaction catalysed:
UDP-glucose + hydroquinone = UDP + hydroquinone-O-beta-D-glucopyranoside
Biological process:
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glycosyltransferase 72B1 Chain: A
Molecule details ›
Chain: A
Length: 480 amino acids
Theoretical weight: 52.99 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9M156 (Residues: 1-480; Coverage: 100%)
Gene names: At4g01070, F2N1.15, UGT72B1
Sequence domains: UDP-glucoronosyl and UDP-glucosyl transferase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 47.807Å b: 94.335Å c: 57.059Å
α: 90° β: 110.31° γ: 90°
R-values:
R R work R free
0.16 0.158 0.198
Expression system: Escherichia coli