› 2v7w

X-ray diffraction
1.9Å resolution

X-ray crystal structure of 5'-fluorodeoxyadenosine synthase s158g mutant complexed with 5'-fluorodeoxyadenosin

Released:
DOI: 10.2210/pdb2v7w/pdb
PDB 2v7w coloured by chain and viewed from the front.
PDB 2v7w coloured by chain and viewed from the side.
PDB 2v7w coloured by chain and viewed from the top.
Source organism: Streptomyces cattleya
Primary publication:
Mechanism of enzymatic fluorination in Streptomyces cattleya.
Zhu X, Robinson DA, McEwan AR, O'Hagan D, Naismith JH
J Am Chem Soc 129 14597-604 (2007)
PMID: 17985882

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + fluoride = 5'-deoxy-5'-fluoroadenosine + L-methionine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5'-fluoro-5'-deoxy-adenosine synthase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 299 amino acids
Theoretical weight: 32.37 KDa
Source organism: Streptomyces cattleya
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q70GK9 (Residues: 1-299; Coverage: 100%)
Gene name: flA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 5FD 3 x 5FD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2221
Unit cell:
a: 75.854Å b: 129.256Å c: 184.485Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.221
Expression system: Escherichia coli BL21(DE3)

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Citations

15 review citations

Biocatalysis in radiochemistry: Enzymatic incorporation of PET radionuclides into molecules of biomedical interest.
da Silva et al. (2018)
14 more