› 2v3c

X-ray diffraction
2.5Å resolution

Crystal structure of the SRP54-SRP19-7S.S SRP RNA complex of M. jannaschii

Released:
DOI: 10.2210/pdb2v3c/pdb
PDB entry 2v3c coloured by chain, front view.
PDB entry 2v3c coloured by chain, side view.
PDB entry 2v3c coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Interaction of signal-recognition particle 54 GTPase domain and signal-recognition particle RNA in the free signal-recognition particle.
Hainzl T, Huang S, Sauer-Eriksson AE
Proc Natl Acad Sci U S A 104 14911-6 (2007)
PMID: 17846429

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176434 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Signal recognition particle 19 kDa protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 87 amino acids
Theoretical weight: 10.38 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58440 (Residues: 1-87; Coverage: 100%)
Gene names: MJ1034, srp19
Sequence domains: SRP19 protein
Structure domains: Signal recognition particle, SRP19-like subunit
Signal recognition particle 54 kDa protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 432 amino acids
Theoretical weight: 48.19 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57565 (Residues: 2-427; Coverage: 95%)
Gene names: MJ0101, srp54
Sequence domains:
Structure domains:
7S RNA Chains: M, N
Molecule details ›
Chains: M, N
Length: 96 nucleotides
Theoretical weight: 31.16 KDa
Sequence domains: Bacterial small signal recognition particle RNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 70.286Å b: 129.402Å c: 163.417Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.244 0.294
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Protein targeting by the signal recognition particle.
Grudnik et al. (2009)
7 more

12 mentions without citation

Signal recognition particle: an essential protein-targeting machine.
Akopian et al. (2013)
11 more