› 2v0w

X-ray diffraction
1.7Å resolution

N- and C-terminal helices of oat LOV2 (404-546) are involved in light- induced signal transduction (cryo-trapped light structure of LOV2 (404-546))

Released:
DOI: 10.2210/pdb2v0w/pdb
PDB 2v0w coloured by chain and viewed from the front.
PDB 2v0w coloured by chain and viewed from the side.
PDB 2v0w coloured by chain and viewed from the top.
Source organism: Avena sativa
Entry authors: Halavaty AS, Moffat K

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NPH1-1 Chain: A
Molecule details ›
Chain: A
Length: 146 amino acids
Theoretical weight: 16.85 KDa
Source organism: Avena sativa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O49003 (Residues: 404-546; Coverage: 16%)
Gene name: NPH1-1
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments

2 bound ligands:
Ligand FMN 1 x FMN
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 35.565Å b: 56.021Å c: 66.507Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.16 0.215
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

36 review citations

Optogenetics in bacteria - applications and opportunities.
Lindner et al. (2022)
35 more

9 mentions without citation

CRAC channel-based optogenetics.
Nguyen et al. (2018)
8 more