› 2v0n

X-ray diffraction
2.71Å resolution

ACTIVATED RESPONSE REGULATOR PLED IN COMPLEX WITH C-DIGMP AND GTP- ALPHA-S

Released:
DOI: 10.2210/pdb2v0n/pdb
PDB entry 2v0n coloured by chain, front view.
PDB entry 2v0n coloured by chain, side view.
PDB entry 2v0n coloured by chain, top view.
Source organism: Caulobacter vibrioides CB15
Primary publication:
Structure of BeF3- -modified response regulator PleD: implications for diguanylate cyclase activation, catalysis, and feedback inhibition.
Wassmann P, Chan C, Paul R, Beck A, Heerklotz H, Jenal U, Schirmer T
Structure 15 915-27 (2007)
PMID: 17697997

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110720 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Response regulator PleD Chains: A, B
Molecule details ›
Chains: A, B
Length: 459 amino acids
Theoretical weight: 50.39 KDa
Source organism: Caulobacter vibrioides CB15
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B8GZM2 (Residues: 2-454; Coverage: 100%)
Gene names: CCNA_02546, pleD
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand BEF 2 x BEF
Ligand MG 5 x MG
Ligand C2E 4 x C2E
Ligand GAV 2 x GAV
Ligand SO4 2 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 128.965Å b: 132.557Å c: 88.425Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.215 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

24 review citations

Principles of c-di-GMP signalling in bacteria.
Hengge R. (2009)
23 more

29 mentions without citation

Cyclic di-GMP: the first 25 years of a universal bacterial second messenger.
Römling et al. (2013)
28 more