2uxn

X-ray diffraction
2.72Å resolution

Structural Basis of Histone Demethylation by LSD1 Revealed by Suicide Inactivation

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Lysine-specific histone demethylase 1A Chain: A
Molecule details ›
Chain: A
Length: 666 amino acids
Theoretical weight: 74.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O60341 (Residues: 171-836; Coverage: 78%)
Gene names: AOF2, KDM1, KDM1A, KIAA0601, LSD1
Sequence domains:
Structure domains:
REST corepressor 1 Chain: B
Molecule details ›
Chain: B
Length: 235 amino acids
Theoretical weight: 26.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9UKL0 (Residues: 289-485; Coverage: 41%)
Gene names: KIAA0071, RCOR, RCOR1
Sequence domains: Myb-like DNA-binding domain
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
Histone H3.1 Chain: E
Molecule details ›
Chain: E
Length: 21 amino acids
Theoretical weight: 2.32 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 2-22; Coverage: 15%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: I222
Unit cell:
a: 119.949Å b: 178.646Å c: 234.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.241 0.272
Expression systems:
  • Escherichia coli BL21
  • Not provided