› 2ubp

X-ray diffraction
2Å resolution

Function and Biology Details

Reaction catalysed: 
Urea + H(2)O = CO(2) + 2 NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero nonamer
PDBe Complex ID:
PDB-CPX-154231 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Urease subunit gamma Chain: A
Molecule details ›
Chain: A
Length: 101 amino acids
Theoretical weight: 11.19 KDa
Source organism: Sporosarcina pasteurii
UniProt:
  • Canonical: P41022 (Residues: 1-100; Coverage: 100%)
Gene name: ureA
Sequence domains: Urease, gamma subunit
Structure domains: Urease, gamma-like subunit
Urease subunit beta Chain: B
Molecule details ›
Chain: B
Length: 122 amino acids
Theoretical weight: 13.53 KDa
Source organism: Sporosarcina pasteurii
UniProt:
  • Canonical: P41021 (Residues: 5-126; Coverage: 97%)
Gene name: ureB
Sequence domains: Urease beta subunit
Structure domains: Urease, beta subunit
Urease subunit alpha Chain: C
Molecule details ›
Chain: C
Length: 570 amino acids
Theoretical weight: 61.65 KDa
Source organism: Sporosarcina pasteurii
UniProt:
  • Canonical: P41020 (Residues: 1-27, 29-34, 35-570; Coverage: 100%)
Gene names: NCTC4822_02163, ureC
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand NI 2 x NI
1 modified residue:
Ligand KCX 1 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P6322
Unit cell:
a: 131.357Å b: 131.357Å c: 189.756Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.16 0.16 0.2

Quick links

Citations

16 review citations

Physiological functions of mineral micronutrients (Cu, Zn, Mn, Fe, Ni, Mo, B, Cl).
Hänsch et al. (2009)
15 more

11 mentions without citation

Nonredox nickel enzymes.
Maroney et al. (2014)
10 more