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PHE 18 VARIANT OF TURKEY OVOMUCOID INHIBITOR THIRD DOMAIN COMPLEXED WITH STREPTOMYCES GRISEUS PROTEINASE B

X-ray diffraction
1.75Å resolution
PDB entry 2sgf coloured by chain, front view.
PDB entry 2sgf coloured by chain, side view.
PDB entry 2sgf coloured by chain, top view.
Source organisms:
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Ovomucoid and Streptogrisin-B
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Multimeric state: hetero dimer
Accessible surface area: 9505.6 Å2
Buried surface area: 1520.65 Å2
Dissociation area: 688.55 Å2
Dissociation energy (ΔGdiss): 7.38 kcal/mol
Dissociation entropy (TΔSdiss): 10.09 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-133489

Macromolecules

Streptogrisin-B
Chain: E
Length: 185 amino acids
Theoretical weight: 18.65 KDa
Source organism: Streptomyces griseus
UniProt:
  • Canonical: P00777 (Residues: 115-299; Coverage: 71%)
Gene name: sprB
Pfam: Trypsin
InterPro:
CATH: Trypsin-like serine proteases
SCOP: Prokaryotic proteases
Streptogrisin-B in PDB entry 2sgf, assembly 1, front view.
Streptogrisin-B in PDB entry 2sgf, assembly 1, side view.
Streptogrisin-B in PDB entry 2sgf, assembly 1, top view.
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Ovomucoid
Chain: I
Length: 51 amino acids
Theoretical weight: 5.62 KDa
Source organism: Meleagris gallopavo
Expression system: Escherichia coli
UniProt:
  • Canonical: P68390 (Residues: 135-185; Coverage: 28%)
Pfam: Kazal-type serine protease inhibitor domain
InterPro:
CATH: Wheat Germ Agglutinin (Isolectin 2); domain 1
SCOP: Ovomucoid domain III-like
Ovomucoid in PDB entry 2sgf, assembly 1, front view.
Ovomucoid in PDB entry 2sgf, assembly 1, side view.
Ovomucoid in PDB entry 2sgf, assembly 1, top view.
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