› 2rnf

X-ray diffraction
2.4Å resolution

X-RAY CRYSTAL STRUCTURE OF HUMAN RIBONUCLEASE 4 IN COMPLEX WITH D(UP)

Released:
DOI: 10.2210/pdb2rnf/pdb
PDB entry 2rnf coloured by chain, front view.
PDB entry 2rnf coloured by chain, side view.
PDB entry 2rnf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The three-dimensional structure of human RNase 4, unliganded and complexed with d(Up), reveals the basis for its uridine selectivity.
Terzyan SS, Peracaula R, de Llorens R, Tsushima Y, Yamada H, Seno M, Gomis-Rüth FX, Coll M
J Mol Biol 285 205-14 (1999)
PMID: 9878400

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152682 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 120 amino acids
Theoretical weight: 13.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P34096 (Residues: 29-147; Coverage: 100%)
Gene names: RNASE4, RNS4
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand UM3 2 x UM3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 32.83Å b: 94.33Å c: 40.66Å
α: 90° β: 107.2° γ: 90°
R-values:
R R work R free
0.16 0.16 0.215
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The eight human "canonical" ribonucleases: molecular diversity, catalytic properties, and special biological actions of the enzyme proteins.
Sorrentino S. (2010)
4 more

1 mention without citation

Sequence-specific backbone (1)H, (13)C, and (15)N resonance assignments of human ribonuclease 4.
Gagné et al. (2015)