› 2rdd

X-ray diffraction
3.5Å resolution

X-ray crystal structure of AcrB in complex with a novel transmembrane helix.

Released:
DOI: 10.2210/pdb2rdd/pdb
PDB 2rdd coloured by chain and viewed from the front.
PDB 2rdd coloured by chain and viewed from the side.
PDB 2rdd coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Crystal structure of AcrB in complex with a single transmembrane subunit reveals another twist.
T��rnroth-Horsefield S, Gourdon P, Horsefield R, Brive L, Yamamoto N, Mori H, Snijder A, Neutze R
Structure 15 1663-73 (2007)
PMID: 18073115

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Multidrug efflux pump subunit AcrB Chain: A
Molecule details ›
Chain: A
Length: 1049 amino acids
Theoretical weight: 113.67 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P31224 (Residues: 1-1049; Coverage: 100%)
Gene names: JW0451, acrB, acrE, b0462
Sequence domains: AcrB/AcrD/AcrF family
Structure domains:
Sec translocon accessory complex subunit YajC Chain: B
Molecule details ›
Chain: B
Length: 37 amino acids
Theoretical weight: 4.45 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADZ7 (Residues: 19-55; Coverage: 34%)
Gene names: JW0397, b0407, yajC

Ligands and Environments

1 bound ligand:
Ligand AIC 2 x AIC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: R32
Unit cell:
a: 145.097Å b: 145.097Å c: 511.644Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.279 0.279 0.317

Quick links

Citations

22 review citations

Structural Insights into Transporter-Mediated Drug Resistance in Infectious Diseases.
Kim et al. (2021)
21 more

2 mentions without citation

Structure of the AcrAB-TolC multidrug efflux pump.
Du et al. (2014)
1 more