2rcs

X-ray diffraction
2.1Å resolution

IMMUNOGLOBULIN 48G7 GERMLINE FAB-AFFINITY MATURATION OF AN ESTEROLYTIC ANTIBODY

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
IMMUNOGLOBULIN 48G7 GERMLINE FAB Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.46 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
Structure domains: Immunoglobulins
Immunoglobulin heavy constant gamma 1 Chain: H
Molecule details ›
Chain: H
Length: 217 amino acids
Theoretical weight: 23.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01857 (Residues: 1-103; Coverage: 31%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 66.16Å b: 75.55Å c: 86.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.259
Expression system: Escherichia coli BL21