› 2rcn

X-ray diffraction
2.25Å resolution

Crystal Structure of the Ribosomal interacting GTPase YjeQ from the Enterobacterial species Salmonella Typhimurium.

Released:
DOI: 10.2210/pdb2rcn/pdb
PDB 2rcn coloured by chain and viewed from the front.
PDB 2rcn coloured by chain and viewed from the side.
PDB 2rcn coloured by chain and viewed from the top.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Structure of the ribosomal interacting GTPase YjeQ from the enterobacterial species Salmonella typhimurium.
Nichols CE, Johnson C, Lamb HK, Lockyer M, Charles IG, Hawkins AR, Stammers DK
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 63 922-8 (2007)
PMID: 18007041

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small ribosomal subunit biogenesis GTPase RsgA Chain: A
Molecule details ›
Chain: A
Length: 358 amino acids
Theoretical weight: 39.9 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8ZKB0 (Residues: 2-350; Coverage: 100%)
Gene names: STM4349, engC, rsgA, yjeQ
Sequence domains: RsgA GTPase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand MG 1 x MG
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ DW
Spacegroup: P3121
Unit cell:
a: 92.838Å b: 92.838Å c: 70.227Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.199 0.267
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The Impact of the Stringent Response on TRAFAC GTPases and Prokaryotic Ribosome Assembly.
Bennison et al. (2019)
1 more

3 mentions without citation

Cryo-electron microscopy structure of the 30S subunit in complex with the YjeQ biogenesis factor.
Jomaa et al. (2011)
2 more