› 2r9h

X-ray diffraction
3.1Å resolution

Crystal Structure of Q207C Mutant of CLC-ec1 in complex with Fab

Released:
DOI: 10.2210/pdb2r9h/pdb
PDB entry 2r9h coloured by chain, front view.
PDB entry 2r9h coloured by chain, side view.
PDB entry 2r9h coloured by chain, top view.
Source organisms:
Entry authors: Nguitragool W, Miller C

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211177 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H(+)/Cl(-) exchange transporter ClcA Chains: A, B
Molecule details ›
Chains: A, B
Length: 444 amino acids
Theoretical weight: 47.33 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P37019 (Residues: 17-460; Coverage: 94%)
Gene names: JW5012, b0155, clcA, eriC, yadQ
Sequence domains: Voltage gated chloride channel
Structure domains: Clc chloride channel
Fab fragment Chains: C, E
Molecule details ›
Chains: C, E
Length: 221 amino acids
Theoretical weight: 23.69 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Fab fragment Chains: D, F
Molecule details ›
Chains: D, F
Length: 211 amino acids
Theoretical weight: 23.09 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 233.099Å b: 98.358Å c: 171.922Å
α: 90° β: 131.77° γ: 90°
R-values:
R R work R free
0.262 0.261 0.28
Expression system: Escherichia coli BL21

Quick links