X-ray diffraction
1.7Å resolution

Crystal structure of PurP from Pyrococcus furiosus complexed with AMP

Source organism: Pyrococcus furiosus
Entry authors: Zhang Y, White RH, Ealick SE

Function and Biology Details

Reaction catalysed:
ATP + formate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = ADP + phosphate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 334 amino acids
Theoretical weight: 38.35 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q8U0R7 (Residues: 1-334; Coverage: 100%)
Gene names: PF1517, purP
Sequence domains:
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: R32
Unit cell:
a: 123.205Å b: 123.205Å c: 376.298Å
α: 90° β: 90° γ: 120°
R R work R free
0.167 0.165 0.186
Expression system: Escherichia coli BL21(DE3)