2r2b › Citations

Exploration of specificity in germline monoclonal antibody recognition of a range of natural and synthetic epitopes.

Brooks CL, Müller-Loennies S, Brade L, Kosma P, Hirama T, MacKenzie CR, Brade H, Evans SV
J Mol Biol 377 450-68 (2008)
Related entries: 2r1w, 2r1x, 2r1y, 2r23, 2r2e, 2r2h, 3bpc

Cited: 25 times
EuropePMC logo PMID: 18272175

Abstract

To explore the molecular basis of antigen recognition by germline antibodies, we have determined to high resolution the structures of the near-germline monoclonal antibody S25-2 in complex with seven distinct carbohydrate antigens based on the bacterial sugar 3-deoxy-alpha-D-manno-oct-2-ulosonic acid (Kdo). In contrast to previous findings, the inherited germline Kdo monosaccharide binding site is not restricted to this bacterial sugar but is able to accommodate an array of substitutions and chemical modifications of Kdo, including naturally occurring antigens containing the related monosaccharide d-glycero-alpha-d-talo-oct-2-ulosonic acid as well as nonterminal Kdo residues. However, we show by surface plasmon resonance and ELISA how antibody S25-2 specificity is so dependent on the context in which the antigen is presented that a free disaccharide displays strong binding while the same lipid-A-bound disaccharide does not bind. These structures provide insight into how inherited germline genes code for immunoglobulins of limited flexibility that are capable of binding a range of epitopes from which affinity-matured antibodies are generated.

Articles - 2r2b mentioned but not cited (1)

  1. Predicting binding sites from unbound versus bound protein structures. Clark JJ, Orban ZJ, Carlson HA. Sci Rep 10 15856 (2020)


Reviews citing this publication (3)

  1. Grading the commercial optical biosensor literature-Class of 2008: 'The Mighty Binders'. Rich RL, Myszka DG. J. Mol. Recognit. 23 1-64 (2010)
  2. Antibody recognition of carbohydrate epitopes†. Haji-Ghassemi O, Blackler RJ, Martin Young N, Evans SV. Glycobiology 25 920-952 (2015)
  3. Antibody promiscuity: Understanding the paradigm shift in antigen recognition. Kaur H, Salunke DM. IUBMB Life 67 498-505 (2015)

Articles citing this publication (21)

  1. Spatially addressed combinatorial protein libraries for recombinant antibody discovery and optimization. Mao H, Graziano JJ, Chase TM, Bentley CA, Bazirgan OA, Reddy NP, Song BD, Smider VV. Nat. Biotechnol. 28 1195-1202 (2010)
  2. Structural and immunochemical analysis of the lipopolysaccharide from Acinetobacter lwoffii F78 located outside Chlamydiaceae with a Chlamydia-specific lipopolysaccharide epitope. Hanuszkiewicz A, Hübner G, Vinogradov E, Lindner B, Brade L, Brade H, Debarry J, Heine H, Holst O. Chemistry 14 10251-10258 (2008)
  3. Analysis of cross-reactive and specific anti-carbohydrate antibodies against lipopolysaccharide from Chlamydophila psittaci. Gerstenbruch S, Brooks CL, Kosma P, Brade L, Mackenzie CR, Evans SV, Brade H, Müller-Loennies S. Glycobiology 20 461-472 (2010)
  4. Antibodies raised against chlamydial lipopolysaccharide antigens reveal convergence in germline gene usage and differential epitope recognition. Brooks CL, Müller-Loennies S, Borisova SN, Brade L, Kosma P, Hirama T, Mackenzie CR, Brade H, Evans SV. Biochemistry 49 570-581 (2010)
  5. Structural insights into parallel strategies for germline antibody recognition of lipopolysaccharide from Chlamydia. Evans DW, Müller-Loennies S, Brooks CL, Brade L, Kosma P, Brade H, Evans SV. Glycobiology 21 1049-1059 (2011)
  6. The role of CDR H3 in antibody recognition of a synthetic analog of a lipopolysaccharide antigen. Brooks CL, Blackler RJ, Sixta G, Kosma P, Müller-Loennies S, Brade L, Hirama T, MacKenzie CR, Brade H, Evans SV. Glycobiology 20 138-147 (2010)
  7. Groove-type recognition of chlamydiaceae-specific lipopolysaccharide antigen by a family of antibodies possessing an unusual variable heavy chain N-linked glycan. Haji-Ghassemi O, Müller-Loennies S, Saldova R, Muniyappa M, Brade L, Rudd PM, Harvey DJ, Kosma P, Brade H, Evans SV. J. Biol. Chem. 289 16644-16661 (2014)
  8. Synthesis of a neoglycoconjugate containing a Chlamydophila psittaci-specific branched Kdo trisaccharide epitope. Kosma P, Hofinger A, Müller-Loennies S, Brade H. Carbohydr. Res. 345 704-708 (2010)
  9. Surface Plasmon Resonance Analysis Shows an IgG-Isotype-Specific Defect in ABO Blood Group Antibody Formation in Patients with Common Variable Immunodeficiency. Fischer MB, Wolfram W, Binder CJ, Böhmig GA, Wahrmann M, Eibl MM, Wolf HM. Front Immunol 6 211 (2015)
  10. Defining the recognition elements of Lewis Y-reactive antibodies. Saha S, Pashov A, Siegel ER, Murali R, Kieber-Emmons T. PLoS ONE 9 e104208 (2014)
  11. Pneumococcal Polysaccharide Vaccination Elicits IgG Anti-A/B Blood Group Antibodies in Healthy Individuals and Patients with Type I Diabetes Mellitus. Wolfram W, Sauerwein KM, Binder CJ, Eibl-Musil N, Wolf HM, Fischer MB. Front Immunol 7 493 (2016)
  12. Therapeutic Antibodies to Ganglioside GD2 Evolved from Highly Selective Germline Antibodies. Sterner E, Peach ML, Nicklaus MC, Gildersleeve JC. Cell Rep 20 1681-1691 (2017)
  13. A Structural Model for the Ligand Binding of Pneumococcal Serotype 3 Capsular Polysaccharide-Specific Protective Antibodies. Ozdilek A, Huang J, Babb R, Paschall AV, Middleton DR, Duke JA, Pirofski LA, Mousa JJ, Avci FY. mBio 12 e0080021 (2021)
  14. Antigen binding by conformational selection in near-germline antibodies. Blackler RJ, Müller-Loennies S, Pokorny-Lehrer B, Legg MSG, Brade L, Brade H, Kosma P, Evans SV. J Biol Chem 298 101901 (2022)
  15. Selective Recognition of Carbohydrate Antigens by Germline Antibodies Isolated from AID Knockout Mice. DeLaitsch AT, Pridgen JR, Tytla A, Peach ML, Hu R, Farnsworth DW, McMillan AK, Flanagan N, Temme JS, Nicklaus MC, Gildersleeve JC. J Am Chem Soc 144 4925-4941 (2022)
  16. Single-chain antibody-fragment M6P-1 possesses a mannose 6-phosphate monosaccharide-specific binding pocket that distinguishes N-glycan phosphorylation in a branch-specific manner†. Blackler RJ, Evans DW, Smith DF, Cummings RD, Brooks CL, Braulke T, Liu X, Evans SV, Müller-Loennies S. Glycobiology 26 181-192 (2016)
  17. Structural Basis for Antibody Recognition of Lipid A: INSIGHTS TO POLYSPECIFICITY TOWARD SINGLE-STRANDED DNA. Haji-Ghassemi O, Müller-Loennies S, Rodriguez T, Brade L, Kosma P, Brade H, Evans SV. J. Biol. Chem. 290 19629-19640 (2015)
  18. Synthesis and antigenic properties of C-7-modified Kdo mono- and disaccharide ligands and Kdo disaccharide interresidue lactones. Sixta G, Wimmer K, Hofinger A, Brade H, Kosma P. Carbohydr. Res. 344 1660-1669 (2009)
  19. Update on carbohydrate-containing antibacterial agents. Schitter G, Wrodnigg TM. Expert Opin Drug Discov 4 315-356 (2009)
  20. A novel Arg H52/Tyr H33 conservative motif in antibodies: A correlation between sequence of antibodies and antigen binding. Petrov A, Arzhanik V, Makarov G, Koliasnikov O. J Bioinform Comput Biol 14 1650019 (2016)
  21. Chicken novel leukocyte immunoglobulin-like receptor subfamilies B1 and B3 are transcriptional regulators of major histocompatibility complex class I genes and signaling pathways. Truong AD, Hong Y, Lee J, Lee K, Tran HTT, Dang HV, Nguyen VK, Lillehoj HS, Hong YH. Asian-australas. J. Anim. Sci. 32 614-628 (2019)


Quick links