› 2qv8

X-ray diffraction
2Å resolution

Structure of the minor pseudopilin EpsH from the Type 2 Secretion System of Vibrio cholerae

Released:
DOI: 10.2210/pdb2qv8/pdb
PDB 2qv8 coloured by chain and viewed from the front.
PDB 2qv8 coloured by chain and viewed from the side.
PDB 2qv8 coloured by chain and viewed from the top.
Source organism: Vibrio cholerae
Primary publication:
Structure of the minor pseudopilin EpsH from the Type 2 secretion system of Vibrio cholerae.
Yanez ME, Korotkov KV, Abendroth J, Hol WG
J. Mol. Biol. 377 91-103 (2008)
PMID: 18241884

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II secretion system protein H Chains: A, B
Molecule details ›
Chains: A, B
Length: 159 amino acids
Theoretical weight: 18.21 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P45774 (Residues: 36-194; Coverage: 82%)
Gene names: VC_2729, epsH
Sequence domains: Type II transport protein GspH
Structure domains: minor pseudopilin epsh domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 53.349Å b: 70.449Å c: 85.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.212
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Virulence Regulation and Innate Host Response in the Pathogenicity of Vibrio cholerae.
Ramamurthy et al. (2020)
6 more

6 mentions without citation

Structural insights into the Type II secretion nanomachine.
McLaughlin et al. (2012)
5 more