› 2quk

X-ray diffraction
2.8Å resolution

Crystal structures of human tryptophanyl-tRNA synthetase in complex with ATP(putative)

Released:
DOI: 10.2210/pdb2quk/pdb
PDB 2quk coloured by chain and viewed from the front.
PDB 2quk coloured by chain and viewed from the side.
PDB 2quk coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Catalytic mechanism of the tryptophan activation reaction revealed by crystal structures of human tryptophanyl-tRNA synthetase in different enzymatic states.
Shen N, Zhou M, Yang B, Yu Y, Dong X, Ding J
OpenAccess logo Nucleic Acids Res. 36 1288-99 (2008)
PMID: 18180246

Function and Biology Details

Reaction catalysed: 
ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan--tRNA ligase, cytoplasmic Chain: A
Molecule details ›
Chain: A
Length: 477 amino acids
Theoretical weight: 54.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23381 (Residues: 1-471; Coverage: 100%)
Gene names: IFI53, WARS, WARS1, WRS
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P622
Unit cell:
a: 235.6Å b: 235.6Å c: 48.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.267 0.267 0.29
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Unique roles of tryptophanyl-tRNA synthetase in immune control and its therapeutic implications.
Jin M. (2019)
1 more

2 mentions without citation

Crystal structures of Plasmodium falciparum cytosolic tryptophanyl-tRNA synthetase and its potential as a target for structure-guided drug design.
Koh et al. (2013)
1 more