› 2qs8

X-ray diffraction
2.33Å resolution

Crystal structure of a Xaa-Pro dipeptidase with bound methionine in the active site

Released:
DOI: 10.2210/pdb2qs8/pdb
PDB entry 2qs8 coloured by chain, front view.
PDB entry 2qs8 coloured by chain, side view.
PDB entry 2qs8 coloured by chain, top view.
Source organism: Alteromonas macleodii
Primary publication:
Functional annotation of two new carboxypeptidases from the amidohydrolase superfamily of enzymes.
Xiang DF, Xu C, Kumaran D, Brown AC, Sauder JM, Burley SK, Swaminathan S, Raushel FM
Biochemistry 48 4567-76 (2009)
PMID: 19358546

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo octamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125585 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidohydrolase-related domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 418 amino acids
Theoretical weight: 46.01 KDa
Source organism: Alteromonas macleodii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: K7N5L1 (Residues: 1-418; Coverage: 100%)
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand MET 2 x MET
1 modified residue:
Ligand MSE 26 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I4
Unit cell:
a: 144.846Å b: 144.846Å c: 101.029Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Metagenomics and the protein universe.
Godzik A. (2011)
1 more

4 mentions without citation

Gulosibacter molinativorax ON4T molinate hydrolase, a novel cobalt-dependent amidohydrolase.
Duarte et al. (2011)
3 more