2qpl

X-ray diffraction
2.1Å resolution

Crystal structure of calf spleen purine nucleoside phosphorylase complexed to a novel purine analogue

Released:

Function and Biology Details

Reaction catalysed:
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 282 amino acids
Theoretical weight: 31.43 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P55859 (Residues: 3-284; Coverage: 98%)
Gene names: NP, PNP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P213
Unit cell:
a: 93.442Å b: 93.442Å c: 93.442Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.218