› 2qdj

X-ray diffraction
2Å resolution

Crystal structure of the Retinoblastoma protein N-domain provides insight into tumor suppression, ligand interaction and holoprotein architecture

Released:
DOI: 10.2210/pdb2qdj/pdb
PDB entry 2qdj coloured by chain, front view.
PDB entry 2qdj coloured by chain, side view.
PDB entry 2qdj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the retinoblastoma protein N domain provides insight into tumor suppression, ligand interaction, and holoprotein architecture.
Hassler M, Singh S, Yue WW, Luczynski M, Lakbir R, Sanchez-Sanchez F, Bader T, Pearl LH, Mittnacht S
Mol Cell 28 371-85 (2007)
PMID: 17996702

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139024 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinoblastoma-associated protein Chain: A
Molecule details ›
Chain: A
Length: 304 amino acids
Theoretical weight: 35.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06400 (Residues: 52-355; Coverage: 33%)
Gene name: RB1
Sequence domains: Domain of unknown function (DUF3452)
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C222
Unit cell:
a: 72.296Å b: 106.649Å c: 98.191Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.228 0.276
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Conserved functions of the pRB and E2F families.
van den Heuvel et al. (2008)
14 more

9 mentions without citation

Molecular mechanisms underlying RB protein function.
Dick et al. (2013)
8 more