X-ray diffraction
1.8Å resolution

Crystal structure of ferric G248T cytochrome P450cam

Source organism: Pseudomonas putida
Entry authors: von Koenig K, Makris TM, Sligar SD, Schlichting I

Function and Biology Details

Reaction catalysed:
(+)-camphor + reduced putidaredoxin + O(2) = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Camphor 5-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 421 amino acids
Theoretical weight: 47.59 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P00183 (Residues: 1-415; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 64.09Å b: 64.59Å c: 106.26Å
α: 90° β: 90° γ: 90°
R R work R free
0.219 0.219 0.264
Expression system: Escherichia coli BL21(DE3)