X-ray diffraction
1.9Å resolution

Crystal structure of human cytochrome P450 46A1 in complex with cholesterol-3-sulphate


Function and Biology Details

Reaction catalysed:
Cholesterol + [reduced NADPH--hemoprotein reductase] + O(2) = (24S)-cholest-5-ene-3-beta,24-diol + [oxidized NADPH--hemoprotein reductase] + H(2)O
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Cholesterol 24-hydroxylase Chain: A
Molecule details ›
Chain: A
Length: 456 amino acids
Theoretical weight: 52.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli DH5[alpha]
  • Canonical: Q9Y6A2 (Residues: 51-500; Coverage: 90%)
Gene names: CYP46, CYP46A1
Structure domains: Cytochrome P450

Ligands and Environments

Cofactor: Ligand HEM 1 x HEM
3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: I4122
Unit cell:
a: 121.85Å b: 121.85Å c: 144.03Å
α: 90° β: 90° γ: 90°
R R work R free
0.187 0.183 0.216
Expression system: Escherichia coli DH5[alpha]