› 2q86

X-ray diffraction
1.85Å resolution

Structure of the mouse invariant NKT cell receptor Valpha14

Released:
DOI: 10.2210/pdb2q86/pdb
PDB entry 2q86 coloured by chain, front view.
PDB entry 2q86 coloured by chain, side view.
PDB entry 2q86 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structures of mouse CD1d-iGb3 complex and its cognate Valpha14 T cell receptor suggest a model for dual recognition of foreign and self glycolipids.
Zajonc DM, Savage PB, Bendelac A, Wilson IA, Teyton L
J Mol Biol 377 1104-16 (2008)
PMID: 18295796

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134770 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
T-cell receptor alpha chain constant Chains: A, C
Molecule details ›
Chains: A, C
Length: 229 amino acids
Theoretical weight: 25.4 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P01849 (Residues: 1-115; Coverage: 85%)
Gene name: Trac
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins
T-cell receptor beta-2 chain C region Chains: B, D
Molecule details ›
Chains: B, D
Length: 254 amino acids
Theoretical weight: 28.07 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P01851 (Residues: 1-136; Coverage: 79%)
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer
1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 65.293Å b: 150.518Å c: 65.814Å
α: 90° β: 117.04° γ: 90°
R-values:
R R work R free
0.18 0.179 0.223
Expression system: Drosophila melanogaster

Quick links

Citations

35 review citations

T cell antigen receptor recognition of antigen-presenting molecules.
Rossjohn et al. (2015)
34 more

3 mentions without citation

Differential recognition of CD1d-alpha-galactosyl ceramide by the V beta 8.2 and V beta 7 semi-invariant NKT T cell receptors.
Pellicci et al. (2009)
2 more