› 2q7a

X-ray diffraction
2.1Å resolution

Crystal structure of the cell surface heme transfer protein Shp

Released:
DOI: 10.2210/pdb2q7a/pdb
PDB entry 2q7a coloured by chain, front view.
PDB entry 2q7a coloured by chain, side view.
PDB entry 2q7a coloured by chain, top view.
Source organism: Streptococcus pyogenes
Primary publication:
Bis-methionyl coordination in the crystal structure of the heme-binding domain of the streptococcal cell surface protein Shp.
Aranda R, Worley CE, Liu M, Bitto E, Cates MS, Olson JS, Lei B, Phillips GN
J Mol Biol 374 374-83 (2007)
PMID: 17920629

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-170415 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell surface protein Shp haem-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 152 amino acids
Theoretical weight: 16.62 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q06A48 (Residues: 30-180; Coverage: 52%)
Gene name: shp
Sequence domains: Cell surface heme-binding protein Shp
Structure domains: Immunoglobulin-like

Ligands and Environments

2 bound ligands:
Ligand HEM 4 x HEM
Ligand GOL 9 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D, APS BEAMLINE 21-ID-D
Spacegroup: P65
Unit cell:
a: 82.317Å b: 82.317Å c: 106.054Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 0.163 0.222
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Recent developments in understanding the iron acquisition strategies of gram positive pathogens.
Sheldon et al. (2015)
3 more

6 mentions without citation

Pathway for heme uptake from human methemoglobin by the iron-regulated surface determinants system of Staphylococcus aureus.
Zhu et al. (2008)
5 more