2q5e

X-ray diffraction
2.51Å resolution

Crystal structure of human carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2

Released:

Function and Biology Details

Reaction catalysed:
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 187 amino acids
Theoretical weight: 21.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14595 (Residues: 87-271; Coverage: 68%)
Gene names: CTDSP2, NIF2, OS4, SCP2
Sequence domains: NLI interacting factor-like phosphatase
Structure domains: HAD superfamily/HAD-like

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 108.643Å b: 117.637Å c: 170.134Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.216 0.278
Expression system: Escherichia coli BL21(DE3)