2q0c

X-ray diffraction
2.2Å resolution

Terminal uridylyl transferase 4 from Trypanosoma brucei with bound CTP

Released:
Source organism: Trypanosoma brucei
Primary publication:
Dual role of the RNA substrate in selectivity and catalysis by terminal uridylyl transferases.
Proc. Natl. Acad. Sci. U.S.A. 104 14634-9 (2007)
PMID: 17785418

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PAP-associated domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 353 amino acids
Theoretical weight: 39.88 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q381M1 (Residues: 1-333; Coverage: 100%)
Gene name: Tb11.01.7300
Sequence domains: Cid1 family poly A polymerase

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 81.166Å b: 42.157Å c: 106.809Å
α: 90° β: 93.66° γ: 90°
R-values:
R R work R free
0.234 0.232 0.281
Expression system: Escherichia coli BL21(DE3)