2pyl

X-ray diffraction
2.2Å resolution

Phi29 DNA polymerase complexed with primer-template DNA and incoming nucleotide substrates (ternary complex)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 575 amino acids
Theoretical weight: 66.72 KDa
Source organism: Bacillus virus phi29
Expression system: Escherichia coli
UniProt:
  • Canonical: P03680 (Residues: 1-575; Coverage: 100%)
Gene name: 2
Sequence domains: DNA polymerase type B, organellar and viral
Structure domains:
5'-d(GACTGCTTAC(2DA))-3' Chain: X
Molecule details ›
Chain: X
Length: 11 nucleotides
Theoretical weight: 3.32 KDa
Source organism: Bacillus virus phi29
Expression system: Not provided
5'-d(CTGACGAATGTACA)-3' Chain: Y
Molecule details ›
Chain: Y
Length: 14 nucleotides
Theoretical weight: 4.29 KDa
Source organism: Bacillus virus phi29
Expression system: Not provided

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 58.922Å b: 78.199Å c: 157.813Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.191 0.253
Expression systems:
  • Escherichia coli
  • Not provided