2pwq

X-ray diffraction
1.9Å resolution

Crystal structure of a putative ubiquitin conjugating enzyme from Plasmodium yoelii

Released:
Source organism: Plasmodium yoelii
Entry authors: Qiu W, Dong A, Hassanali A, Lin L, Brokx S, Altamentova S, Hills T, Lew J, Ravichandran M, Kozieradzki I, Zhao Y, Schapira M, Edwards AM, Arrowsmith CH, Weigelt J, Sundstrom M, Bochkarev A, Hui R, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E2 ubiquitin-conjugating enzyme Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 24.49 KDa
Source organism: Plasmodium yoelii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7RP52 (Residues: 8-202; Coverage: 97%)
Gene name: PY01609
Sequence domains:
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P3121
Unit cell:
a: 48.556Å b: 48.556Å c: 179.502Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.269 0.267 0.308
Expression system: Escherichia coli