› 2pwf

X-ray diffraction
1.8Å resolution

Crystal structure of the MutB D200A mutant in complex with glucose

Released:
DOI: 10.2210/pdb2pwf/pdb
PDB entry 2pwf coloured by chain, front view.
PDB entry 2pwf coloured by chain, side view.
PDB entry 2pwf coloured by chain, top view.
Source organism: Burkholderia ubonensis subsp. mesacidophila
Primary publication:
Trehalulose synthase native and carbohydrate complexed structures provide insights into sucrose isomerization.
Ravaud S, Robert X, Watzlawick H, Haser R, Mattes R, Aghajari N
J Biol Chem 282 28126-36 (2007)
PMID: 17597061

Function and Biology Details

Reaction catalysed: 
Sucrose = 6-O-alpha-D-glucopyranosyl-D-fructofuranose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173875 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 556 amino acids
Theoretical weight: 63.77 KDa
Source organism: Burkholderia ubonensis subsp. mesacidophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2PS28 (Residues: 29-584; Coverage: 99%)
Gene name: mutB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand BGC 4 x BGC
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P1
Unit cell:
a: 63.98Å b: 85.81Å c: 122.14Å
α: 81.77° β: 81.43° γ: 89.94°
R-values:
R R work R free
0.198 0.198 0.238
Expression system: Escherichia coli

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Citations

5 review citations

α-Amylase: an enzyme specificity found in various families of glycoside hydrolases.
Janeček et al. (2014)
4 more