› 2pvf

X-ray diffraction
1.8Å resolution

Crystal Structure of Tyrosine Phosphorylated Activated FGF Receptor 2 (FGFR2) Kinase Domain in Complex with ATP Analog and Substrate Peptide

Released:
DOI: 10.2210/pdb2pvf/pdb
PDB entry 2pvf coloured by chain, front view.
PDB entry 2pvf coloured by chain, side view.
PDB entry 2pvf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A molecular brake in the kinase hinge region regulates the activity of receptor tyrosine kinases.
Chen H, Ma J, Li W, Eliseenkova AV, Xu C, Neubert TA, Miller WT, Mohammadi M
Mol Cell 27 717-30 (2007)
PMID: 17803937

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149427 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fibroblast growth factor receptor 2 Chain: A
Molecule details ›
Chain: A
Length: 334 amino acids
Theoretical weight: 38.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P21802 (Residues: 458-778; Coverage: 40%)
Gene names: BEK, FGFR2, KGFR, KSAM
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:
Fibroblast growth factor receptor 2 Chain: B
Molecule details ›
Chain: B
Length: 15 amino acids
Theoretical weight: 1.78 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P21802 (Residues: 764-778; Coverage: 2%)
Gene names: BEK, FGFR2, KGFR, KSAM

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ACP 1 x ACP
1 modified residue:
Ligand PTR 3 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 56.353Å b: 78.436Å c: 84.839Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.251 0.251 0.265
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

42 review citations

Cell signaling by receptor tyrosine kinases.
Lemmon et al. (2010)
41 more

33 mentions without citation

Molecular mechanisms of fibroblast growth factor signaling in physiology and pathology.
Belov et al. (2013)
32 more