2puq

X-ray diffraction
2.05Å resolution

Crystal structure of active site inhibited coagulation factor VIIA in complex with soluble tissue factor

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Factor VII light chain Chain: L
Molecule details ›
Chain: L
Length: 94 amino acids
Theoretical weight: 10.26 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P08709 (Residues: 109-202; Coverage: 21%)
Gene name: F7
Sequence domains: EGF-like domain
Structure domains: Laminin
Factor VII heavy chain Chain: H
Molecule details ›
Chain: H
Length: 254 amino acids
Theoretical weight: 28.1 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P08709 (Residues: 213-466; Coverage: 57%)
Gene name: F7
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Tissue factor Chain: T
Molecule details ›
Chain: T
Length: 204 amino acids
Theoretical weight: 23.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13726 (Residues: 38-241; Coverage: 78%)
Gene name: F3
Structure domains: Immunoglobulins
TRP-TYR-THR-ARG CHLOROMETHYLKETONE INHIBITOR Chain: I
Molecule details ›
Chain: I
Length: 5 amino acids
Theoretical weight: 660 Da

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 78.312Å b: 68.827Å c: 78.732Å
α: 90° β: 90.74° γ: 90°
R-values:
R R work R free
0.231 0.228 0.273
Expression systems:
  • Cricetulus griseus
  • Escherichia coli