X-ray diffraction
2.1Å resolution

Human MAK3 homolog in complex with CoA

Source organism: Homo sapiens
Entry authors: Walker JR, Schuetz A, Antoshenko T, Wu H, Bernstein G, Loppnau P, Weigelt J, Sundstrom M, Arrowsmith CH, Edwards AM, Bochkarev A, Plotnikov AN, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed:
Acetyl-CoA + an N-terminal-L-methionyl-L-alanyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-alanyl-[protein] + CoA
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
N-alpha-acetyltransferase 50 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 170 amino acids
Theoretical weight: 19.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9GZZ1 (Residues: 2-169; Coverage: 99%)
Gene names: MAK3, NAA50, NAT13, NAT5
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase

Ligands and Environments

Cofactor: Ligand COA 3 x COA
No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 47.643Å b: 102.913Å c: 67.157Å
α: 90° β: 105.81° γ: 90°
R R work R free
0.201 0.197 0.265
Expression system: Escherichia coli BL21(DE3)