› 2pse

X-ray diffraction
2.5Å resolution

Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis

Released:
DOI: 10.2210/pdb2pse/pdb
PDB entry 2pse coloured by chain, front view.
PDB entry 2pse coloured by chain, side view.
PDB entry 2pse coloured by chain, top view.
Source organism: Renilla reniformis
Primary publication:
Crystal structures of the luciferase and green fluorescent protein from Renilla reniformis.
Loening AM, Fenn TD, Gambhir SS
J Mol Biol 374 1017-28 (2007)
PMID: 17980388

Function and Biology Details

Reaction catalysed: 
(1a) coelenterazine h + O(2) = coelenterazine h dioxetanone
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151045 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Coelenterazine h 2-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 318 amino acids
Theoretical weight: 37 KDa
Source organism: Renilla reniformis
Expression system: Escherichia coli
UniProt:
  • Canonical: P27652 (Residues: 3-311; Coverage: 99%)
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand IMD 1 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P61
Unit cell:
a: 119.443Å b: 119.443Å c: 48.048Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.191 0.232
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
16 more

3 mentions without citation

Crystal structures of the luciferase and green fluorescent protein from Renilla reniformis.
Loening et al. (2007)
2 more