2pse

X-ray diffraction
2.5Å resolution

Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis

Released:
Source organism: Renilla reniformis

Function and Biology Details

Reaction catalysed:
(1a) coelenterazine h + O(2) = coelenterazine h dioxetanone 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Coelenterazine h 2-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 318 amino acids
Theoretical weight: 37 KDa
Source organism: Renilla reniformis
Expression system: Escherichia coli
UniProt:
  • Canonical: P27652 (Residues: 3-311; Coverage: 99%)
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P61
Unit cell:
a: 119.443Å b: 119.443Å c: 48.048Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.191 0.232
Expression system: Escherichia coli