2pox

X-ray diffraction
1.95Å resolution

Dark state structure of the reversibly switchable fluorescent protein Dronpa

Released:
Source organism: Echinophyllia sp. SC22
Entry authors: Trowitzsch S, Weber G, Wahl MC

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Dronpa Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 255 amino acids
Theoretical weight: 29.25 KDa
Source organism: Echinophyllia sp. SC22
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5TLG6 (Residues: 1-224; Coverage: 100%)
Gene name: Dronpa
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: I222
Unit cell:
a: 72.894Å b: 107.504Å c: 275.663Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.216
Expression system: Escherichia coli