› 2poj

Solution NMR

NMR Solution Structure of the Inhibitor-Free State of Macrophage Metalloelastase (MMP-12)

Released:
DOI: 10.2210/pdb2poj/pdb
PDB entry 2poj coloured by chain, ensemble of 20 models, front view.
PDB entry 2poj coloured by chain, ensemble of 20 models, side view.
PDB entry 2poj coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Solution structure of inhibitor-free human metalloelastase (MMP-12) indicates an internal conformational adjustment.
Bhaskaran R, Palmier MO, Bagegni NA, Liang X, Van Doren SR
J Mol Biol 374 1333-44 (2007)
PMID: 17997411

Function and Biology Details

Reaction catalysed: 
Hydrolysis of soluble and insoluble elastin (1). Specific cleavages are also produced at -Ala(14)-|-Leu- and -Tyr(16)-|-Leu- in the B chain of insulin (2).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153971 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage metalloelastase Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P39900 (Residues: 100-263; Coverage: 36%)
Gene names: HME, MMP12
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: SIMULATED ANNEALING, TORSION ANGLE DYNAMICS, AUTOMATED ANALYSIS OF NOESY DATA AND 3D STRUCTURES.
Expression system: Escherichia coli

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Citations

1 review citation

Matrix metalloproteinase interactions with collagen and elastin.
Van Doren SR. (2015)
6 other articles

7 mentions without citation

MMP-12 catalytic domain recognizes triple helical peptide models of collagen V with exosites and high activity.
Bhaskaran et al. (2008)
6 more