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Crystal structure of the P. abyssi exosome RNase PH ring complexed with ADP in double conformation

X-ray diffraction
2.3Å resolution
PDB entry 2po0 coloured by chain, front view.
PDB entry 2po0 coloured by chain, side view.
PDB entry 2po0 coloured by chain, top view.
Source organism: Pyrococcus abyssi
Assembly composition:
hetero hexamer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Exosome complex
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Multimeric state: hetero hexamer
Accessible surface area: 58666.44 Å2
Buried surface area: 20740.12 Å2
Dissociation area: 3,611.12 Å2
Dissociation energy (ΔGdiss): 21.19 kcal/mol
Dissociation entropy (TΔSdiss): 30.32 kcal/mol
Symmetry number: 3
PDBe Complex ID: PDB-CPX-194159

Macromolecules

Exosome complex component Rrp41
Chain: A
Length: 249 amino acids
Theoretical weight: 27.72 KDa
Source organism: Pyrococcus abyssi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9V119 (Residues: 1-249; Coverage: 100%)
Gene names: PAB0420, PYRAB06100, rrp41
Pfam:
InterPro:
CATH: GHMP Kinase, N-terminal domain
Exosome complex component Rrp41 in PDB entry 2po0, assembly 1, front view.
Exosome complex component Rrp41 in PDB entry 2po0, assembly 1, side view.
Exosome complex component Rrp41 in PDB entry 2po0, assembly 1, top view.
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Exosome complex component Rrp42
Chain: B
Length: 277 amino acids
Theoretical weight: 30.27 KDa
Source organism: Pyrococcus abyssi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9V118 (Residues: 1-274; Coverage: 100%)
Gene names: PAB0421, PYRAB06110, rrp42
Pfam:
InterPro:
CATH: GHMP Kinase, N-terminal domain
Exosome complex component Rrp42 in PDB entry 2po0, assembly 1, front view.
Exosome complex component Rrp42 in PDB entry 2po0, assembly 1, side view.
Exosome complex component Rrp42 in PDB entry 2po0, assembly 1, top view.
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