2pnz

X-ray diffraction
2.14Å resolution

Crystal structure of the P. abyssi exosome RNase PH ring complexed with UDP and GMP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194159 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Exosome complex component Rrp41 Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 27.72 KDa
Source organism: Pyrococcus abyssi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9V119 (Residues: 1-249; Coverage: 100%)
Gene names: PAB0420, PYRAB06100, rrp41
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain
Exosome complex component Rrp42 Chain: B
Molecule details ›
Chain: B
Length: 277 amino acids
Theoretical weight: 30.27 KDa
Source organism: Pyrococcus abyssi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9V118 (Residues: 1-274; Coverage: 100%)
Gene names: PAB0421, PYRAB06110, rrp42
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: P321
Unit cell:
a: 93.81Å b: 93.81Å c: 126.26Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.188 0.255
Expression system: Escherichia coli BL21(DE3)