› 2plv

X-ray diffraction
2.88Å resolution

STRUCTURAL FACTORS THAT CONTROL CONFORMATIONAL TRANSITIONS AND SEROTYPE SPECIFICITY IN TYPE 3 POLIOVIRUS

Released:
DOI: 10.2210/pdb2plv/pdb
PDB entry 2plv coloured by chain, front view.
PDB entry 2plv coloured by chain, side view.
PDB entry 2plv coloured by chain, top view.
Source organism: Human poliovirus 1 Mahoney
Primary publication:
Structural factors that control conformational transitions and serotype specificity in type 3 poliovirus.
Filman DJ, Syed R, Chow M, Macadam AJ, Minor PD, Hogle JM
EMBO J 8 1567-79 (1989)
PMID: 2548847

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero 240-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136699 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Capsid protein VP1 Chain: 1
Molecule details ›
Chain: 1
Length: 302 amino acids
Theoretical weight: 33.38 KDa
Source organism: Human poliovirus 1 Mahoney
UniProt:
  • Canonical: P03300 (Residues: 580-881; Coverage: 14%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP2 Chain: 2
Molecule details ›
Chain: 2
Length: 272 amino acids
Theoretical weight: 30.08 KDa
Source organism: Human poliovirus 1 Mahoney
UniProt:
  • Canonical: P03300 (Residues: 70-341; Coverage: 12%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP3 Chain: 3
Molecule details ›
Chain: 3
Length: 238 amino acids
Theoretical weight: 26.55 KDa
Source organism: Human poliovirus 1 Mahoney
UniProt:
  • Canonical: P03300 (Residues: 342-579; Coverage: 11%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP4 Chain: 4
Molecule details ›
Chain: 4
Length: 68 amino acids
Theoretical weight: 7.39 KDa
Source organism: Human poliovirus 1 Mahoney
UniProt:
  • Canonical: P03300 (Residues: 2-69; Coverage: 3%)
Sequence domains: Picornavirus coat protein (VP4)
Structure domains: Picornavirus coat protein VP4

Ligands and Environments

2 bound ligands:
Ligand SPH 1 x SPH
Ligand MYR 1 x MYR
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 322.94Å b: 358.04Å c: 380.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 not available

Quick links

Citations

28 review citations

Vaccine-derived polioviruses and the endgame strategy for global polio eradication.
Kew et al. (2005)
27 more

28 mentions without citation

Trendspotting in the Protein Data Bank.
Berman et al. (2013)
27 more