Structure analysis

T7 RNA polymerase complexed with a phi10 promoter

X-ray diffraction
2.9Å resolution
Source organism: Escherichia phage T7
Assembly composition:
hetero trimer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero trimer
Accessible surface area: 41700 Å2
Buried surface area: 5000 Å2
Dissociation area: 1,900 Å2
Dissociation energy (ΔGdiss): 10 kcal/mol
Dissociation entropy (TΔSdiss): 13 kcal/mol
Interface energy (ΔGint): -15 kcal/mol
Symmetry number: 1

Macromolecules

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Name: 5'-D(*CP*TP*TP*C*CP*TP*AP*TP*AP*GP*TP*GP*AP*GP*TP*CP*GP*TP*AP*TP*TP*A)-3'
Representative chains: T
Source organism: Escherichia phage T7 [10760]
Expression system: Not provided
Length: 22 nucleotides
Theoretical weight: 6.73 KDa

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Name: 5'-D(*TP*AP*AP*TP*AP*CP*GP*AP*CP*TP*CP*AP*CP*T)-3'
Representative chains: P
Source organism: Escherichia phage T7 [10760]
Expression system: Not provided
Length: 14 nucleotides
Theoretical weight: 4.22 KDa

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