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T7 RNA polymerase complexed with a phi10 promoter

X-ray diffraction
2.9Å resolution
PDB 2pi5 coloured by chain and viewed from the front.
PDB 2pi5 coloured by chain and viewed from the side.
PDB 2pi5 coloured by chain and viewed from the top.
Source organism: Escherichia phage T7
Assembly composition:
hetero trimer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero trimer
Accessible surface area: 41700 Å2
Buried surface area: 5000 Å2
Dissociation area: 1,900 Å2
Dissociation energy (ΔGdiss): 10 kcal/mol
Dissociation entropy (TΔSdiss): 13 kcal/mol
Interface energy (ΔGint): -15 kcal/mol
Symmetry number: 1

Macromolecules

T7 RNA polymerase
Chain: A
Length: 878 amino acids
Theoretical weight: 98.41 KDa
Source organism: Enterobacteria phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P00573 (Residues: 6-883; Coverage: 99%)
Gene name: 1
Pfam:
InterPro:
CATH:
SCOP: T7 RNA polymerase
PDB 2pi5 contains 1 copy of T7 RNA polymerase in assembly 1. This protein is highlighted and viewed from the front.
PDB 2pi5 contains 1 copy of T7 RNA polymerase in assembly 1. This protein is highlighted and viewed from the side.
PDB 2pi5 contains 1 copy of T7 RNA polymerase in assembly 1. This protein is highlighted and viewed from the top.
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DNA chain T
Name: 5'-D(*CP*TP*TP*C*CP*TP*AP*TP*AP*GP*TP*GP*AP*GP*TP*CP*GP*TP*AP*TP*TP*A)-3'
Representative chains: T
Source organism: Escherichia phage T7 [10760]
Expression system: Not provided
Length: 22 nucleotides
Theoretical weight: 6.73 KDa
PDB 2pi5 contains 1 copy of 5'-D(*CP*TP*TP*C*CP*TP*AP*TP*AP*GP*TP*GP*AP*GP*TP*CP*GP*TP*AP*TP*TP*A)-3' in assembly 1. This DNA molecule is highlighted and viewed from the front.
PDB 2pi5 contains 1 copy of 5'-D(*CP*TP*TP*C*CP*TP*AP*TP*AP*GP*TP*GP*AP*GP*TP*CP*GP*TP*AP*TP*TP*A)-3' in assembly 1. This DNA molecule is highlighted and viewed from the side.
PDB 2pi5 contains 1 copy of 5'-D(*CP*TP*TP*C*CP*TP*AP*TP*AP*GP*TP*GP*AP*GP*TP*CP*GP*TP*AP*TP*TP*A)-3' in assembly 1. This DNA molecule is highlighted and viewed from the top.
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DNA chain P
Name: 5'-D(*TP*AP*AP*TP*AP*CP*GP*AP*CP*TP*CP*AP*CP*T)-3'
Representative chains: P
Source organism: Escherichia phage T7 [10760]
Expression system: Not provided
Length: 14 nucleotides
Theoretical weight: 4.22 KDa
PDB 2pi5 contains 1 copy of 5'-D(*TP*AP*AP*TP*AP*CP*GP*AP*CP*TP*CP*AP*CP*T)-3' in assembly 1. This DNA molecule is highlighted and viewed from the front.
PDB 2pi5 contains 1 copy of 5'-D(*TP*AP*AP*TP*AP*CP*GP*AP*CP*TP*CP*AP*CP*T)-3' in assembly 1. This DNA molecule is highlighted and viewed from the side.
PDB 2pi5 contains 1 copy of 5'-D(*TP*AP*AP*TP*AP*CP*GP*AP*CP*TP*CP*AP*CP*T)-3' in assembly 1. This DNA molecule is highlighted and viewed from the top.
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